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Target Analysis / Targeted Metabolic Profiling

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Target Analysis/Classes Profiling

HPLC-API-MS-PDA coupling is perfectly suited for target analysis and classes profiling. High speed RP Chromatography with the use of commercial silica based monolithic C-18 columns offers short time analysis. FINNIGAN LTQ allows to perform qualitative and quantitative analysis in a full scan mode as well as SIM and SRM modes. Online CID fragmentation experiments in both positive and negative modes giving an opportunity for identification/elucidation of unknown detected components as well as verification of the known ones. Parallel UV/VIS data acquisition offers double check for known targets as well as giving valuable information on unknowns and on components that can not be ionized.

Nucleotides separations with LC-ESI-MS-PDA coupling. Standards mixture: A,C,U,G, their MPs,DPs,TPs.

Flavanoids separations with LC-ESI-MS-PDA coupling. Arabidopsis Thalianaleaf acidic water/methanol extract.

             Applied Biosystems                                                  Qtrap 4000 LC-MS/MS System


 

• The ability to quantify known small molecules in a complex background matrix.

• The ability to monitor up to 300 "targets" within a single chromatography run using MRM.

• Targeted Metabolomics: Monitoring small molecules that belong to certain pathways.

 • Installed and coupled with the Waters UPLC system.


Created by admin
Vladimir Tolstikov
Last modified 2009-01-23 12:57
 
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